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fas l  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec fas l
    Fas L, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/CD178+Antibody%2C+anti-human%2C+REAfinity/pm42455847-67-65-67
    Average 93 stars, based on 5 article reviews
    fas l - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: The mineralocorticoid receptor antagonist finerenone enhances cardiovascular recovery upon food intake normalization in obese mice.
    Article Snippet: Endogenous biotin and avidin were blocked for 10 min (Dako Biotin Blocking Kit, USA).

    Article Title: M1-BMDMs with Wnt5a deletion attenuate liver fibrosis by suppression of Wnt5a/Frizzled 2 axis in hepatic progenitors.
    Article Snippet: The antibodies used for immunophenotype analysis of the BMDMs were PE anti-rat CD45 antibody (B256650; BioLegend, Tokyo, Japan); PE-Vio770 anti-rat CD68 antibody (130102726; Miltenyi Biotec, Bergisch Gladbach, Germany); PE anti-rat CD11b antibody (201807; BioLegend, San Diego, CA, USA); Goat anti-rabbit IgG H&L (FITC) (554020) and goat anti-rabbit IgG H&L (PE) antibody (550083) (BD Biosciences, San Jose, CA, USA).

    Incubation:

    Article Title: Compositions and methods for treating pain, inflammation, infection, malaria, and sepsis
    Article Snippet: The flow cytometry events were acquired in a BD FACS Canto II flow cytometer by using BD FACS Diva 8 software (BD Bioscience). .. Laser 405 nm 488 nm 633 nm Filter 425/50 530/30 585/42 660/20 780/60 Experiment nm nm nm nm nm incubation Skin Primary Ab zombi F4/80- Mouse Ly6G- 1-hour on phagocytosis violet FITC anti- APC Cy7 ice GPR37 Second Ab anti- 30 min on mouse- ice Cy5 pMØ depletion Primary Ab zombi F4/80- CD11b- Ly6G- 30 min on violet FITC PE APC Cy7 ice Skin MØ depletion Primary Ab zombi F4/80- Ly6G- 30 min on violet FITC APC Cy7 ice antibody company cat no dilution FACS primary antibody rat-anti CD11b-PE Biolegend 101207 1:200 rat anti mouse-CD68-alexa Biolegend 137011 1:200 fluor 488 human anti mouse-F4/80-APC miltenyl biotec 130-102-379 1:10 rat anti mouse-F4/80-FITC Biolegend 23108 1:200 mouse GPR37 Santa Cruz SC-390110 1:100 or 1:500 rat anti-mouse Ly6G-APCcy7 Biolegend 127623 1:200 rat anti-mouse Ly6G Biolegend 127601 1:1000 FACS second or control antibody rat anti-mouse IgG-FITC eBioscience 11-4011-85 1:200 rat anti-mouse IgG-PE BD Bioscience 51-20415z 1:200 donkey anti-mouse IgG-Cy5 Jackson 715-175-151 1:500 ImmunoResearch goat anti-rabbit igG-APC-Cy7 Santa cruz sc-3847 1:200 Phagocytosis assay. .. 2004, 361, 184-187). pHrodo® Red Zymosan Bioparticles (diameter, 3 μm, Thermo scientific, Catalog: P35364) were rinsed and reconstituted in RPMI medium.

    Article Title: Tumor necrosis factor alpha inhibition improves fetal growth in a rat model of preeclampsia.
    Article Snippet: Whole blood was collected, mixed with RPMI media, and layered on a Ficoll-Hypaque cushion (Lymphoprep, Accurate Chemical & Scientific Corp, Westbury, NY) to isolate lymphocytes. .. For B Cell analysis, one million peripheral blood mononuclear cells (PBMCs) were incubated with anti-rat CD3 (Vioblue, Miltenyi Biotec, San Diego), anti-rat CD45R (APC-Vio 770, Miltenyi Biotec), and anti-rat CD68 (PE Vio 770, Milteni Biotec). ..

    Article Title: The mineralocorticoid receptor antagonist finerenone enhances cardiovascular recovery upon food intake normalization in obese mice
    Article Snippet: Endogenous biotin and avidin were blocked for 10 min (Dako Biotin Blocking Kit, USA). .. Sections were then incubated with a biotinylated primary antibody—rat anti-mouse CD68 (1:200) (130–102-025, Miltenyi, Germany) as previously, before being incubated for 30 min under darkness with secondary reagents SA-Cy5 A647 (1:500) (FP-CA5640, FP-YE5081, Interchim, France). ..

    FACS:

    Article Title: Compositions and methods for treating pain, inflammation, infection, malaria, and sepsis
    Article Snippet: The flow cytometry events were acquired in a BD FACS Canto II flow cytometer by using BD FACS Diva 8 software (BD Bioscience). .. Laser 405 nm 488 nm 633 nm Filter 425/50 530/30 585/42 660/20 780/60 Experiment nm nm nm nm nm incubation Skin Primary Ab zombi F4/80- Mouse Ly6G- 1-hour on phagocytosis violet FITC anti- APC Cy7 ice GPR37 Second Ab anti- 30 min on mouse- ice Cy5 pMØ depletion Primary Ab zombi F4/80- CD11b- Ly6G- 30 min on violet FITC PE APC Cy7 ice Skin MØ depletion Primary Ab zombi F4/80- Ly6G- 30 min on violet FITC APC Cy7 ice antibody company cat no dilution FACS primary antibody rat-anti CD11b-PE Biolegend 101207 1:200 rat anti mouse-CD68-alexa Biolegend 137011 1:200 fluor 488 human anti mouse-F4/80-APC miltenyl biotec 130-102-379 1:10 rat anti mouse-F4/80-FITC Biolegend 23108 1:200 mouse GPR37 Santa Cruz SC-390110 1:100 or 1:500 rat anti-mouse Ly6G-APCcy7 Biolegend 127623 1:200 rat anti-mouse Ly6G Biolegend 127601 1:1000 FACS second or control antibody rat anti-mouse IgG-FITC eBioscience 11-4011-85 1:200 rat anti-mouse IgG-PE BD Bioscience 51-20415z 1:200 donkey anti-mouse IgG-Cy5 Jackson 715-175-151 1:500 ImmunoResearch goat anti-rabbit igG-APC-Cy7 Santa cruz sc-3847 1:200 Phagocytosis assay. .. 2004, 361, 184-187). pHrodo® Red Zymosan Bioparticles (diameter, 3 μm, Thermo scientific, Catalog: P35364) were rinsed and reconstituted in RPMI medium.

    Control:

    Article Title: Compositions and methods for treating pain, inflammation, infection, malaria, and sepsis
    Article Snippet: The flow cytometry events were acquired in a BD FACS Canto II flow cytometer by using BD FACS Diva 8 software (BD Bioscience). .. Laser 405 nm 488 nm 633 nm Filter 425/50 530/30 585/42 660/20 780/60 Experiment nm nm nm nm nm incubation Skin Primary Ab zombi F4/80- Mouse Ly6G- 1-hour on phagocytosis violet FITC anti- APC Cy7 ice GPR37 Second Ab anti- 30 min on mouse- ice Cy5 pMØ depletion Primary Ab zombi F4/80- CD11b- Ly6G- 30 min on violet FITC PE APC Cy7 ice Skin MØ depletion Primary Ab zombi F4/80- Ly6G- 30 min on violet FITC APC Cy7 ice antibody company cat no dilution FACS primary antibody rat-anti CD11b-PE Biolegend 101207 1:200 rat anti mouse-CD68-alexa Biolegend 137011 1:200 fluor 488 human anti mouse-F4/80-APC miltenyl biotec 130-102-379 1:10 rat anti mouse-F4/80-FITC Biolegend 23108 1:200 mouse GPR37 Santa Cruz SC-390110 1:100 or 1:500 rat anti-mouse Ly6G-APCcy7 Biolegend 127623 1:200 rat anti-mouse Ly6G Biolegend 127601 1:1000 FACS second or control antibody rat anti-mouse IgG-FITC eBioscience 11-4011-85 1:200 rat anti-mouse IgG-PE BD Bioscience 51-20415z 1:200 donkey anti-mouse IgG-Cy5 Jackson 715-175-151 1:500 ImmunoResearch goat anti-rabbit igG-APC-Cy7 Santa cruz sc-3847 1:200 Phagocytosis assay. .. 2004, 361, 184-187). pHrodo® Red Zymosan Bioparticles (diameter, 3 μm, Thermo scientific, Catalog: P35364) were rinsed and reconstituted in RPMI medium.

    Phagocytosis Assay:

    Article Title: Compositions and methods for treating pain, inflammation, infection, malaria, and sepsis
    Article Snippet: The flow cytometry events were acquired in a BD FACS Canto II flow cytometer by using BD FACS Diva 8 software (BD Bioscience). .. Laser 405 nm 488 nm 633 nm Filter 425/50 530/30 585/42 660/20 780/60 Experiment nm nm nm nm nm incubation Skin Primary Ab zombi F4/80- Mouse Ly6G- 1-hour on phagocytosis violet FITC anti- APC Cy7 ice GPR37 Second Ab anti- 30 min on mouse- ice Cy5 pMØ depletion Primary Ab zombi F4/80- CD11b- Ly6G- 30 min on violet FITC PE APC Cy7 ice Skin MØ depletion Primary Ab zombi F4/80- Ly6G- 30 min on violet FITC APC Cy7 ice antibody company cat no dilution FACS primary antibody rat-anti CD11b-PE Biolegend 101207 1:200 rat anti mouse-CD68-alexa Biolegend 137011 1:200 fluor 488 human anti mouse-F4/80-APC miltenyl biotec 130-102-379 1:10 rat anti mouse-F4/80-FITC Biolegend 23108 1:200 mouse GPR37 Santa Cruz SC-390110 1:100 or 1:500 rat anti-mouse Ly6G-APCcy7 Biolegend 127623 1:200 rat anti-mouse Ly6G Biolegend 127601 1:1000 FACS second or control antibody rat anti-mouse IgG-FITC eBioscience 11-4011-85 1:200 rat anti-mouse IgG-PE BD Bioscience 51-20415z 1:200 donkey anti-mouse IgG-Cy5 Jackson 715-175-151 1:500 ImmunoResearch goat anti-rabbit igG-APC-Cy7 Santa cruz sc-3847 1:200 Phagocytosis assay. .. 2004, 361, 184-187). pHrodo® Red Zymosan Bioparticles (diameter, 3 μm, Thermo scientific, Catalog: P35364) were rinsed and reconstituted in RPMI medium.

    Cell Analysis:

    Article Title: Tumor necrosis factor alpha inhibition improves fetal growth in a rat model of preeclampsia.
    Article Snippet: Whole blood was collected, mixed with RPMI media, and layered on a Ficoll-Hypaque cushion (Lymphoprep, Accurate Chemical & Scientific Corp, Westbury, NY) to isolate lymphocytes. .. For B Cell analysis, one million peripheral blood mononuclear cells (PBMCs) were incubated with anti-rat CD3 (Vioblue, Miltenyi Biotec, San Diego), anti-rat CD45R (APC-Vio 770, Miltenyi Biotec), and anti-rat CD68 (PE Vio 770, Milteni Biotec). ..



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    Image Search Results


    The effect of fisetin (10 and 20 µM) on Jurkat cell survival, measured using XTT assay. In the presence and absence of Fas receptor inhibitor Met-12. Data represented as mean ± SEM ( n = 3) Statistical analysis carried out using ANOVA followed by Tukey’s post hoc test for significance (* p < 0.05) (*** p < 0.001) (**** p < 0.0001).

    Journal: International Journal of Molecular Sciences

    Article Title: Synergistic Induction of Caspase-8-Mediated Leukaemic Cell Death by Fisetin and Pinocembrin

    doi: 10.3390/ijms27125622

    Figure Lengend Snippet: The effect of fisetin (10 and 20 µM) on Jurkat cell survival, measured using XTT assay. In the presence and absence of Fas receptor inhibitor Met-12. Data represented as mean ± SEM ( n = 3) Statistical analysis carried out using ANOVA followed by Tukey’s post hoc test for significance (* p < 0.05) (*** p < 0.001) (**** p < 0.0001).

    Article Snippet: Cells were treated with the Fas inhibitor Met-12 (CAS No. 1242964-46-0, purchased from MedChemExpress, catalogue number HY-P6440) at concentration of 20 μM and fisetin at concentrations of 10 μM and 20 μM, and combination treatments consisting of MET + fisetin (20 + 10 μM and 20 μM + 20 μM).

    Techniques: XTT Assay